anti runx2 rabbit polyclonal  (Novus Biologicals)

Journal: Biomaterials advances

Article Title: In vitro development and optimization of cell-laden injectable bioprinted gelatin methacryloyl (GelMA) microgels mineralized on the nanoscale.

doi: 10.1016/j.bioadv.2024.213805

Figure Lengend Snippet: Fig. 5. Osteocyte morphology and functionality in mineralized and non-mineralized microgels. A-B and E-F represent the confocal images of non-mineralized (A-B) and mineralized (E-F) samples. Microgels stained with actin (green), DAPI (blue), and SOST (red) and SOST intensity (normalized fluorescence per cell) in mineralized microgels (A-H, K-L). Also, the dendrite length in μm (I) and the number of dendrites per cell (I-J) were not significantly different between mineralized and non-mineralized samples. Statistical differences are represented by **** p < 0.0001 after the One-way ANOVA test post-Turkey’s corrections.

Article Snippet: Cells were permeabilized with 0.1 % Triton X100, blocked with 1.5 % bovine serum albumin in PBS, and incubated at room temperature for 1 h. Samples were then washed in PBS and incubated with the primary antibody (rabbit M.G. da Costa Sousa et al. Biomaterials Advances 159 (2024) 213805 polyclonal SOST antibody (Novus Biologicals, NBP1-77461)) (1:100 dilution), overnight at 4 ◦C.

Techniques: Staining, Fluorescence

Journal: bioRxiv

Article Title: In vitro development and optimization of cell-laden injectable bioprinted gelatin methacryloyl (GelMA) microgels mineralized on the nanoscale

doi: 10.1101/2023.10.10.560919

Figure Lengend Snippet: Osteocyte morphology and functionality in mineralized and non-mineralized microgels. A-B and E-F represent the confocal images of non-mineralized (A-B) and mineralized (E-F) samples. Microgels stained with actin, DAPI, and SOST revels higher cell number and SOST intensity (normalized fluorescence per cell) in mineralized microgels (A-H, K-L). Also, the dendrite length in μm (I) and the number of dendrites per cell (I-J) were not significantly different between mineralized and non-mineralized samples. Statistical differences are represented by **** p<0.0001 after the One-way ANOVA test post-Turkey’s corrections.

Article Snippet: Cells were permeabilized with 0.1% Triton X100, blocked with 1.5% bovine serum albumin in PBS, and incubated at room temperature for 1 h. Samples were then washed in PBS and incubated with the primary antibody (rabbit polyclonal SOST antibody (Novus Biologicals, NBP1-77461)) (1: 100 dilution), overnight at 4 °C.

Techniques: Staining, Fluorescence

Journal: Science advances

Article Title: Adseverin, an actin-binding protein, modulates hypertrophic chondrocyte differentiation and osteoarthritis progression.

doi: 10.1126/sciadv.adf1130

Figure Lengend Snippet: Fig. 6. Repopulation of adseverin-positive chondrocytes attenuated changes in Indian hedgehog and Runx2 expression despite increased proliferation, ap- optosis, collagen type X, and decreased aggrecan and F-actin. Immunohistochemical staining and quantification at 18 weeks of (A and B) adseverin, (C and D) Ihh, and (E and F) Ki67, as well as immunostaining for (G) aggrecan in the articular cartilage of Adseverin−/−and Adseverin+/+ mice. Scale bars, 200 μm. (H) Apoptotic chon- drocytes and the actin cytoskeleton were detected by immunofluorescent staining with TUNEL (green) and phalloidin (red), respectively. DAPI (blue) stains nuclei. Scale bar, 400 μm. Higher-magnification images of the red or white dashed box in regions are in fig. S6. (I) Quantification of TUNEL-positive cells in the articular cartilage of Adseverin−/−and Adseverin+/+ mice. Immunohistochemical staining of hypertrophic markers (J) Runx2 with (K) quantification and (L) collagen type X in the articular cartilage of Adseverin−/−versus Adseverin+/+ mice. Dot plots represent the mean with minimum and maximum values. Statistical analysis was assessed using Student’s unpaired two-tailed t test (B, D, F, I, and K). The number of biological replicates in each group and P values are indicated in the figure.

Article Snippet: Glogauer) (69), human adseverin (1:200; ab223055, Abcam), Ihh (1:100; ab39634, Abcam), Ki67 (1:50; ab15580, Abcam), αSMA (1:200; ab5694, Abcam), Runx2 (1:50; NBP1-77461, Novus Biologicals), MMP13 (1:200; 18165-1-AP, Proteintech), and collagen type X (1:1000; ab260040, Abcam).

Techniques: Expressing, Immunohistochemical staining, Staining, Immunostaining, TUNEL Assay, Two Tailed Test